Bacterial Environmental Genomic Efforts
with Dr. Ed Delong, we have screened a large contig Antarctic environmental
DNA library for bacterial diversity. Several clones with bacterial
rDNA genes have been selected for complete sequencing. In order
to sequence the large clones (40-50 kb) they had to be subcloned
first. We used a strategy including nebulization followed by blunt
ligation and cloning into E. coli, then picked clones to fill ten
96-well plates for each library.
View subcloning project table (PDF file: 43KB) listing Phylogenetic relatives of large contig clones and progress to date. Table will open in a separate window.