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Sequence assembly and Gene identificationShotgun CloningEnvironmental Library ScreeningMicroarraysLaboratory-based StudiesField Research ProgramProject Summary

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Environmental Library ScreeningShotgun CloningSequence assembly and Gene identificationMicroarrays

GenEx2 kicked off officially in Alison Murray's Molecular Microbial Ecology lab at the DRI in June 2001. The first task stemmed from previous work that had been initiated during a previous field season in the Antarctic Peninsula in 1996. During that season bacterioplankton from thousands of liters of seawater was concentrated, the DNA was extracted and a large contig DNA library was constructed by Alison's Ph.D. advisor, Ed DeLong. Ed's group has since sequenced one of the archaeal clones from this library (Beja` et al. 2002. Appl. Environ. Microbiol. 68(1):335-345).

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